Cross-cultural adaptation and validation of a questionnaire on eating habits and physical activity of university students in confinement due to coronavirus disease.

OBJECTIVE: The purpose of the current study was to cross-culturally adapt and validate an online questionnaire to assess eating habits and physical activity of university students under confinement due to coronavirus disease (COVID-19). DESIGN: Generation of a cross-sectional online survey to university students conducted during confinement due to COVID-19. The study was divided into two phases. SETTINGS: Students, Chile. PARTICIPANTS: Phase 1 considered the process of translation and back translation, expert panel, cultural adaptation and the generation of a pilot to validate a preliminary format of the questionnaire. In Phase 2, information from the instrument was collected from two hundred and sixty-eight university students, ages 16 to 30 years old, with a mean age of 21·6 (3·3) The major proportion of participants were female (82 %). RESULTS: The adapted questionnaire was statistically validated in three dimensions: (A) eating habits and behaviours during quarantine, (B) perception of risk and (C) physical activity changes during the quarantine. The reliability of Cronbach's α for dimensions A, B and C was 0·59, 0·85 and 0·97, respectively. The complete questionnaire obtained 0·61 in internal consistency and 0·61 (0·58-0·67) ICC reliability. A statistically significant positive correlation matrix was observed. CONCLUSIONS: This questionnaire is a practical tool to obtain accurate information about the relation of COVID-19 confinement on people's eating habits and physical activity. Therefore, it could contribute to establishing appropriate strategies to prevent negative effects on people's health.

Adaptación y validación de cuestionario para medir hábitos alimentarios en educación superior / Adaptation and validation of a questionnaire to measure food habits in higher education

RESUMEN Validar instrumentos de medición de datos es fundamental para constituir conocimiento certero y objetivo respecto a los hábitos en ingesta alimentaria. El artículo pretende adaptar y validar para el contexto de educación superior un cuestionario que originalmente es de atención primaria, y que se transforme en un insumo válido para medir hábitos alimentarios en estudiantes universitarios. Basados en un cuestionario validado por Pino, Díaz y López en el 2011, se ajustaron las preguntas para un contexto de educación terciaria. El instrumento se aplicó a una muestra estratificada de 405 estudiantes de pregrado de una universidad perteneciente al Consejo de Rectores de Chile (CRUCH) de la región del Maule. Los datos fueron procesados mediante el paquete estadístico SPSS 20, y contó con los respectivos resguardos éticos mediante consentimiento informado firmado. Siguiendo los pertinentes análisis estadísticos para la validación del instrumento, podemos dar cuenta de un cuestionario confiable y aceptable (α= 0,815), estableciéndose 10 dimensiones con 35 preguntas. Se concluye un instrumento válido y adaptado para ser aplicado en estudiantes con el fin de identificar sus hábitos alimentarios en educación superior.

ABSTRACT Validating data measurement instruments is essential to establish accurate and objective knowledge regarding dietary habits. This article aims to adapt and validate a questionnaire to measure dietary habits originally designed for the primary care setting for use in the context of higher education. Based on a questionnaire validated by Pino, Díaz and López in 2011, the questions were adjusted for a tertiary education context. The instrument was applied to a sample of 405 undergraduate students of a university belonging to the Council of Rectors of Chile (CRUCH) of the Maule region, stratified by college year. Data were analysed using SPSS 20; signed informed consent was obtained. Following the relevant statistical analysis for the validation of an instrument, 10 dimensions with 35 questions were established. Results indicated that the instrument is trustworthy and acceptable (α= 0.815). We conclude that the instrument is a valid adaptation and can be applied to higher education students in order to identify their eating habits.

Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum.

BACKGROUND: The present investigation evaluated 4 different solvent compositions for their relative capacity to extract total phenolic and total flavonoid (TF) components of the leaves, trunks, and stems of Bucida buceras L. (Combretaceae), and the stems of Phoradendron californicum (Viscaceae), plus mesquite and oak species endemic to the Southwestern United States, northern Mexico, and tropical regions of Central and South America, as well as to profile the composition of these plant materials and to measure their antioxidant capacity. METHODS: The total phenolic content of plant material used in the present investigation was measured using the Folin-Ciocalteau assay. Total flavonoids were assayed by AlCl3 and 2,4-dinitrophenylhydrazin colorimetry. Nitroblue tetrazolium was utilized for scavenging of superoxide anion, and in vitro antioxidant activity was evaluated using the 2, 2-diphenyl-1-picrylhydrazyl and Ferric Reducing/Antioxidant Power assays. RESULTS: Phytochemical screening of each plant extract evaluated revealed the following major results: (1) No evidence of alkaloids for each of the extraction phases tested was detected in the hexanic, ethanolic, or aqueous phases of Bucida buceras and Phoradendron californicum (oak and mesquite); (2) Analysis of the hexane phase of B. buceras and P. californicum (mesquite) extracts revealed the presence of carotenes, triterpenes/steroids, and lactonic groups; (3) Analysis of the ethanol and aqueous extraction phases for both plants revealed the presence of a diverse range of compounds, including tripterpenes/steroids, lactonics groups, saponins, phenols/tannins, amines and/or amino acids, and flavonoids/anthocyanins; and (4) The highest total phenolic and flavonoid content were observed in P. californicum (oak): 523.886 ± 51.457 µg GAE/mg extract and 409.651 ± 23.091 µg/mg of extract for methanol and aqueous fractions, respectively. The highest flavonoid content was 237.273 ± 21.250 µg PNE/mg extract in the acetone extract of Bucida buceras stems; while the flavonol content (260.685 ± 23.031 µg CE/mg extract) was higher in the ethanol extract of P. californicum (oak). The acetone extract of B. buceras trunk extract showed the highest levels of DPPH radical-scavenging activity (IC50 = 4.136 ± 0.446 µg/mL) and reducing power (4928.392 ± 281.427 µM AAE/mg extract). The highest superoxide radical scavenging activity (IC50) was 55.249 ± 9.829 µg/mL, observed in acetone extracts of B. buceras leaves. CONCLUSIONS: The results of the present investigation demonstrated the effects of extraction solvent on phenolic and flavonoid content yield-and antioxidant activities by Bucida buceras and Phoradendron californicum. The present investigation further revealed that Bucida buceras exhibited optimal antioxidant capacity when acetone was used as extraction solvent; and the highest yield of phenols and flavonoids were obtained from the P. californicum oak, using methanol and aqueous solvents, respectively.

Neumonía necrotizante y sepsis en el síndrome de dificultad respiratoria del adulto / Necrotizing pneumonia and sepsis of adult respiratory distress syndrome

Se estudian 91 pacientes con criterios diagnósticos de síndrome de dificultad respiratoria del adulto.La mortalidad total grupo fue de 51%. La incidencia de sepsis fue del 69%. La neumonía necrotizante se presentó en 32% y se manifestó con mayor frecuencia en pacientes que permanecieron intubados y sometidos a ventilación mecánica por tiempo prolongado. En este grupo la mortalidad se elevó al 66%. Los gérmenes aislados mas frecuentes fueron seudomona, enterobacter, klebsiella y acinetobacter